Anti-Ago2 / eIF2C2 antibody - ChIP Grade
| Name | Anti-Ago2 / eIF2C2 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab57113 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | ChIPseq WB ICC/IF ICC/IF IHC-P IP FC ChIP ICC/IF |
| Species Reactivities | Chicken, Human, Zebrafish |
| Antigen | Recombinant fragment, corresponding to amino acids 483-859 of Human Ago2/ eIF2C2 (UniProt: Q9UKV8) |
| Description | Mouse Monoclonal |
| Gene | AGO2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ago2 / eIF2C2 antibody (ab57113) used in immunofluorescence at 10ug/ml on HeLa cells.
Ago2 / eIF2C2 antibody (ab57113) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.
![Overlay histogram showing HeLa cells stained with ab57113 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57113, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/3624_Ago2-eIF2C2-Primary-antibodies-ab57113-6.jpg)
Overlay histogram showing HeLa cells stained with ab57113 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57113, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
The RNA helicase RHAU (DHX36) suppresses expression of the transcription factor - The RNA helicase RHAU (DHX36) suppresses expression of the transcription factor
Booy EP, Howard R, Marushchak O, Ariyo EO, Meier M, Novakowski SK, Deo SR, Dzananovic E, Stetefeld J, McKenna SA. Nucleic Acids Res. 2014 Mar;42(5):3346-61.
Differential RISC association of endogenous human microRNAs predicts their - Differential RISC association of endogenous human microRNAs predicts their
Flores O, Kennedy EM, Skalsky RL, Cullen BR. Nucleic Acids Res. 2014 Apr;42(7):4629-39.
Analysis of the mRNA targetome of microRNAs expressed by Marek's disease virus. - Analysis of the mRNA targetome of microRNAs expressed by Marek's disease virus.
Parnas O, Corcoran DL, Cullen BR. MBio. 2014 Jan 21;5(1):e01060-13.
Evolutionary conservation of primate lymphocryptovirus microRNA targets. - Evolutionary conservation of primate lymphocryptovirus microRNA targets.
Skalsky RL, Kang D, Linnstaedt SD, Cullen BR. J Virol. 2014 Feb;88(3):1617-35.
Importin 8 regulates the transport of mature microRNAs into the cell nucleus. - Importin 8 regulates the transport of mature microRNAs into the cell nucleus.
Wei Y, Li L, Wang D, Zhang CY, Zen K. J Biol Chem. 2014 Apr 11;289(15):10270-5.
Mammalian microtubule P-body dynamics are mediated by nesprin-1. - Mammalian microtubule P-body dynamics are mediated by nesprin-1.
Rajgor D, Mellad JA, Soong D, Rattner JB, Fritzler MJ, Shanahan CM. J Cell Biol. 2014 May 26;205(4):457-75.
Derivation and characterization of Dicer- and microRNA-deficient human cells. - Derivation and characterization of Dicer- and microRNA-deficient human cells.
Bogerd HP, Whisnant AW, Kennedy EM, Flores O, Cullen BR. RNA. 2014 Jun;20(6):923-37.
Argonaute-2 promotes miR-18a entry in human brain endothelial cells. - Argonaute-2 promotes miR-18a entry in human brain endothelial cells.
Ferreira R, Santos T, Amar A, Gong A, Chen TC, Tahara SM, Giannotta SL, Hofman FM. J Am Heart Assoc. 2014 May 16;3(3):e000968.
Argonaute 2 in cell-secreted microvesicles guides the function of secreted miRNAs - Argonaute 2 in cell-secreted microvesicles guides the function of secreted miRNAs
Lv Z, Wei Y, Wang D, Zhang CY, Zen K, Li L. PLoS One. 2014 Jul 29;9(7):e103599.
Analysis of nuclear RNA interference in human cells by subcellular fractionation - Analysis of nuclear RNA interference in human cells by subcellular fractionation
Gagnon KT, Li L, Janowski BA, Corey DR. Nat Protoc. 2014 Sep;9(9):2045-60.