Anti-AGRP antibody

• Supplier: Abcam
• Catalog: ab113481
• Prices: $379.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Human
• Antigen: Synthetic peptide from internal sequence residues of Human AGRP (NM_001138
• Description: Rabbit Polyclonal
• Gene: AGRP
• Conjugate: Unconjugated

Product images

ICC/IF image of ab113481 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab113481 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

All lanes : Anti-AGRP antibody (ab113481) at 1/500 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal testis lysate

Product References

Hypothalamic KLF4 mediates leptin's effects on food intake via AgRP. - Hypothalamic KLF4 mediates leptin's effects on food intake via AgRP.

Imbernon M, Sanchez-Rebordelo E, Gallego R, Gandara M, Lear P, Lopez M, Dieguez C, Nogueiras R. Mol Metab. 2014 Apr 15;3(4):441-51.

Hyperglycemia enhances the proliferation of non-tumorigenic and malignant mammary - Hyperglycemia enhances the proliferation of non-tumorigenic and malignant mammary

Lopez R, Arumugam A, Joseph R, Monga K, Boopalan T, Agullo P, Gutierrez C, Nandy S, Subramani R, de la Rosa JM, Lakshmanaswamy R. PLoS One. 2013 Nov 18;8(11):e79708.