All lanes : Anti-AGXT antibody [EPR13232] (ab178708) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : Human fetal liver lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling AGXT with ab178708 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AGXT with ab178708 at 1/100 dilution.
Western blot analysis on immunoprecipitation pellet from (1) Human fetal liver cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab170891 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.