Anti-AKR1C1/AKR1C2 antibody [4B6AF3]

• Supplier: Abcam
• Catalog: ab131375
• Prices: $376.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone: 4B6AF3
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human, Rabbit, Bovine
• Antigen: Purified protein cocktail
• Description: Mouse Monoclonal
• Gene: AKR1C1
• Conjugate: Unconjugated

Product images

All lanes : Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375) at 2 µg/mlLane 1 : Molecular weight marker Lane 2 : HeLa Cell Lysate at 15 µgLane 3 : HepG2 Cell Lysate at 15 µgLane 4 : Human Liver Homogenate (HLH) at 10 µgLane 5 : Human Heart Homogenate (HHH) at 10 µgLane 6 : Mouse Liver Homogenate (MLH) at 10 µgLane 7 : Rat Liver Homogenate (RLH) at 10 µgLane 8 : Rat Heart Homogenate (RHH) at 10 µgSecondaryGoat anti-Mouse AP at 1/3000 dilution

Immunofluorescence analysis of AKR1C1 in HeLa cells, using ab131375 at 5 µg/ml. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were then incubated with ab131375 for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the cytosol.