Anti-ALDH3A1 antibody
| Name | Anti-ALDH3A1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab76976 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide (Human): a 13 amino acid peptide from near the C terminus of human ALDH3A1 |
| Description | Rabbit Polyclonal |
| Gene | ALDH3A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab76976 stained A549 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76976, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
Lane 1 : Anti-ALDH3A1 antibody (ab76976) at 1 µg/mlLane 2 : Anti-ALDH3A1 antibody (ab76976) at 2 µg/mlLane 1 : human stomach lysate Lane 2 : human stomach lysate Lysates/proteins at 15 µg per lane.
IHC image of ALDH3A1 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76976, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Evaluation of global differential gene and protein expression in primary - Evaluation of global differential gene and protein expression in primary
Hou A, Lan W, Law KP, Khoo SC, Tin MQ, Lim YP, Tong L. PLoS One. 2014 May 13;9(5):e97402.
A novel aldehyde dehydrogenase-3 activator (Alda-89) protects submandibular gland - A novel aldehyde dehydrogenase-3 activator (Alda-89) protects submandibular gland
Xiao N, Cao H, Chen CH, Kong CS, Ali R, Chan C, Sirjani D, Graves E, Koong A, Giaccia A, Mochly-Rosen D, Le QT. Clin Cancer Res. 2013 Aug 15;19(16):4455-64.
In vitro organic nitrate bioactivation to nitric oxide by recombinant aldehyde - In vitro organic nitrate bioactivation to nitric oxide by recombinant aldehyde
Lin S, Page NA, Fung SM, Fung HL. Nitric Oxide. 2013 Nov 30;35:137-43.