Immunoprecipitation image of ab129728. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain. Antibody ab129728 immunocaptured 39.4 kDa ALDOB only from rat liver homogenate (RLH), not from rat brain homogenate (RBH) nor rat skeletal muscle homogenate (RSMH). Gel stained with Coomassie brilliant blue G. Lane1: ladder Lane2: rat brain homogenate lysate Lane3: rat liver homogenate lysate Lane4: rat skeletal muscle homogenate lysate
Immunoprecipitation image of ab129728. ALDOB antibody immunocaptured 39.4 kDa ALDOB from human liver homogenate (HLH), Rat liver homogenate (RLH) and Mouse liver homogenate (MLH). Gel stained with Coomassie brilliant blue G. Lane 1: Ladder Lane 2: Human liver homogenate lysate Lane 3: Rat liver homogenate lysate Lane 4: Mouse liver homogenate lysate
Immunocytochemistry image of ab129728 stained HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab129728 at 5 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) AlexaFluor® 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Antigen retrieval step is recommended for a better signal. The target protein locates to the cytoplasm.
Flow cytometry using ab129728. Hela cells were stained with 5 µg/mL ab129728 antibody (blue) or no primary antibody control (red) and analyzed by flow cytometry.