Anti-Aldolase antibody (HRP) (ab181662) at 1/1000 dilution + A293 whole cell lysatedeveloped using the ECL technique
Immunoprecipitation and Western Blot analysis.300 µl aliquots of whole anti-aldolase antiserum were used to precipitate varying amounts of purified aldolase and precipitates with controls were compared by SDS-PAGE and Western blot. Samples shown in the image are:1. Purified aldolase2. 300 µl antiserum with no antigen (negative control)3. 300 µl antiserum with ~100 µl aldolase (2.5 mg/ml)4. 300 µl antiserum with ~200 µl aldolase (2.5 mg/ml)
Immunoprecipitation was performed with 300 μl of anti-Aldolase antiserum and an equal volume of varied amounts (diluted from a stock solution of ~2.5 mg/ml) of purified aldolase in PBS. 1. Purified Aldolase (1μg)2. Precipitation negative control (antiserum + No antigen)3. 300 μl antiserum + 20 μl Aldolase4. 300 μl antiserum + 80 μl Aldolase5. 300 μl antiserum + 120 μl Aldolase6. 300 μl antiserum + 150 μl Aldolase7. 300 μl antiserum + 300 μl Aldolase