Anti-Alkaline Phosphatase antibody
| Name | Anti-Alkaline Phosphatase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab354 |
| Prices | $394.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ELISA |
| Species Reactivities | Escherichia coli |
| Antigen | Full length protein (E |
| Description | Rabbit Polyclonal |
| Gene | iap |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab354 staining Alkaline Phosphatase in E.coli recombinant protein Mach1 by indirect ELISA. Samples were blocked with 5%milk for 3 hours at room temperature and incubated with primary antibody (1/15000 in TBST + 5% Milk) for 90 minutes. An HRP-conjugated goat polyclonal to Rabbit IgG (1/30000) was used as secondary antibody.See Abreview
All lanes : Anti-Alkaline Phosphatase antibody (ab354) at 1/8000 dilutionLane 1 : Escherichia coli Recombinant protein (Mach1)Lane 2 : Escherichia coli Recombinant protein (Mach1)Lysates/proteins at 0.5 µg per lane.SecondaryAn HRP-conjugated anti-rabbit IgG (whole molecule) at 1/16000 dilutiondeveloped using the ECL techniqueObserved band size : 90 kDa (why is the actual band size different from the predicted?)Exposure time : 2 secondsImage courtesy of an anonymous Abreview.
Product References
The Hsp60-(p.V98I) mutation associated with hereditary spastic paraplegia SPG13 - The Hsp60-(p.V98I) mutation associated with hereditary spastic paraplegia SPG13
Bross P, Naundrup S, Hansen J, Nielsen MN, Christensen JH, Kruhoffer M, Palmfeldt J, Corydon TJ, Gregersen N, Ang D, Georgopoulos C, Nielsen KL. J Biol Chem. 2008 Jun 6;283(23):15694-700.
Membrane-association determinants of the omega-amino acid monooxygenase PvdA, a - Membrane-association determinants of the omega-amino acid monooxygenase PvdA, a
Imperi F, Putignani L, Tiburzi F, Ambrosi C, Cipollone R, Ascenzi P, Visca P. Microbiology. 2008 Sep;154(Pt 9):2804-13.
The elastin-binding protein of Staphylococcus aureus (EbpS) is expressed at the - The elastin-binding protein of Staphylococcus aureus (EbpS) is expressed at the
Downer R, Roche F, Park PW, Mecham RP, Foster TJ. J Biol Chem. 2002 Jan 4;277(1):243-50. Epub 2001 Oct 29.