All lanes : Anti-Alkyl-DHAP synthase antibody [EPR13120] - N-terminal (ab184186) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : SW480 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
All lanes : Anti-Alkyl-DHAP synthase antibody [EPR13120] - N-terminal (ab184186) at 1/2000 dilutionLane 1 : K562 cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling Alkyl-DHAP synthase with ab184186 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling Alkyl-DHAP synthase with ab184186 at a 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 diluiton.
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling Alkyl-DHAP synthase with ab184186 at a 1/100 dilution.