Anti-Alpha 1 Acid Glycoprotein antibody
| Name | Anti-Alpha 1 Acid Glycoprotein antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58291 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 18-202 of Human Alpha 1 Acid Glycoprotein |
| Description | Mouse Monoclonal |
| Gene | ORM1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab58291 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab58291 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alpha 1 Acid Glycoprotein antibody (ab58291) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human stomach carcinoma tissue.
