Anti-alpha 1 Antitrypsin antibody
| Name | Anti-alpha 1 Antitrypsin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab9373 |
| Prices | $390.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P ELISA |
| Species Reactivities | Human, Pig, Mammalian |
| Antigen | Alpha-1-antitrypsin isolated from human serum |
| Description | Rabbit Polyclonal |
| Gene | SERPINA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab9373 staining Human liver. Staining is localised to the cytoplasm and protein is secreted.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ICC/IF image of ab9373 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9373, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab9373 staining alpha 1 Antitrypsin in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Product References
Age-related macular degeneration-associated silent polymorphisms in HtrA1 impair - Age-related macular degeneration-associated silent polymorphisms in HtrA1 impair
Jacobo SM, Deangelis MM, Kim IK, Kazlauskas A. Mol Cell Biol. 2013 May;33(10):1976-90.
Parameters Affecting Image-guided, Hydrodynamic Gene Delivery to Swine Liver. - Parameters Affecting Image-guided, Hydrodynamic Gene Delivery to Swine Liver.
Kamimura K, Suda T, Zhang G, Aoyagi Y, Liu D. Mol Ther Nucleic Acids. 2013 Oct 15;2:e128.
Image-guided, intravascular hydrodynamic gene delivery to skeletal muscle in - Image-guided, intravascular hydrodynamic gene delivery to skeletal muscle in
Kamimura K, Zhang G, Liu D. Mol Ther. 2010 Jan;18(1):93-100.