Anti-alpha 1 Antitrypsin antibody
| Name | Anti-alpha 1 Antitrypsin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab129354 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Human, Chimpanzee, Monkey, Ape, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human alpha 1 Antitrypsin |
| Description | Rabbit Polyclonal |
| Gene | SERPINA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of ab129354 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab129354, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-alpha 1 Antitrypsin antibody (ab129354) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.