Anti-alpha 1 Antitrypsin antibody (HRP)

• Supplier: Abcam
• Catalog: ab191350
• Prices: $384.00
• Sizes: 100 µg
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ELISA IHC-F Immunomicroscopy DB
• Species Reactivities: Human
• Description: Goat Polyclonal
• Gene: SERPINA1
• Conjugate: HRP

Product images

Anti-alpha 1 Antitrypsin antibody (HRP) (ab191350) at 1/5000 dilution + Human Plasma Total Protein at 10 µgdeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab191350 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab191350, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96931, DyLight® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Combination therapy utilizing shRNA knockdown and an optimized resistant - Combination therapy utilizing shRNA knockdown and an optimized resistant

Li C, Xiao P, Gray SJ, Weinberg MS, Samulski RJ. Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14258-63. doi:

Preclinical evaluation of a recombinant adeno-associated virus vector expressing - Preclinical evaluation of a recombinant adeno-associated virus vector expressing

Chulay JD, Ye GJ, Thomas DL, Knop DR, Benson JM, Hutt JA, Wang G, Humphries M, Flotte TR. Hum Gene Ther. 2011 Feb;22(2):155-65.

Relationship of proteinases and proteinase inhibitors with microbial presence in - Relationship of proteinases and proteinase inhibitors with microbial presence in

Davies PL, Spiller OB, Beeton ML, Maxwell NC, Remold-O'Donnell E, Kotecha S. Thorax. 2010 Mar;65(3):246-51.

An efficient rHSV-based complementation system for the production of multiple - An efficient rHSV-based complementation system for the production of multiple

Kang W, Wang L, Harrell H, Liu J, Thomas DL, Mayfield TL, Scotti MM, Ye GJ, Veres G, Knop DR. Gene Ther. 2009 Feb;16(2):229-39.

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Mahadeva R, Atkinson C, Li Z, Stewart S, Janciauskiene S, Kelley DG, Parmar J, Pitman R, Shapiro SD, Lomas DA. Am J Pathol. 2005 Feb;166(2):377-86.