Anti-alpha Actinin 4 antibody
| Name | Anti-alpha Actinin 4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab59468 |
| Prices | $395.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Mouse, Human, Rat |
| Antigen | Synthetic peptide derived from N terminal region of human alpha Actinin 4 |
| Description | Rabbit Polyclonal |
| Gene | ACTN4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-alpha Actinin 4 antibody (ab59468) at 1/1000 dilution + Whole cell lysates prepared from mouse embryonic fibroblasts at 10 µgSecondaryHRP conjugated goat polyclonal to rabbit IgG at 1/3000 dilutionPerformed under reducing conditions.
ab59468 staining alpha Actinin 4 from mouse embryonic fibroblasts by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed prior to blocking in 2% serum for 2 hours at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 18 hours at 4°C. Alexa fluor® 488 goat polyclonal to mouse Ig, diluted 1/1000 was used as the secondary antibody. In image, green is actinin staining and blue is DAPI is DNA staining.See Abreview
IHC image of ab59468 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59468, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.