Anti-alpha Actinin 4 antibody [EPR2533(2)] (HRP)

• Supplier: Abcam
• Catalog: ab199072
• Prices: $425.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR2533(2)
• Applications: IHC-P WB
• Species Reactivities: Human, Mouse, Rat
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human alpha Actinin 4 aa 1-100 (N terminal)
• Description: Rabbit Monoclonal
• Gene: ACTN4
• Conjugate: HRP

Product images

All lanes : Anti-alpha Actinin 4 antibody [EPR2533(2)] (HRP) (ab199072) at 1/5000 dilutionLane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

IHC image of alpha Actinin 4 staining in a section of formalin-fixed paraffin-embedded normal human adrenal gland tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab199072 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre