Anti-alpha Actinin antibody [MAC 276]
| Name | Anti-alpha Actinin antibody [MAC 276] |
|---|---|
| Supplier | Abcam |
| Catalog | ab50599 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | MAC 276 |
| Applications | FC ICC/IF ICC/IF IHC-P Electron microscopy WB |
| Species Reactivities | Rat, Chicken, Human, Drosophila |
| Antigen | Flight muscle extract from Lethocerus indicus (Waterbug) |
| Description | Rat Monoclonal |
| Gene | ACTN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of alpha Actin staining in Human normal bladder formalin fixed paraffin embedded tissue section. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins at 98°C. The section was incubated with ab50599, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. The section was counterstained with haematoxylin and mounted with DPX.
ICC/IF image of ab68826 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab50599 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HeLa cells stained with ab50599 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab50599, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/5609_alpha-Actinin-Primary-antibodies-ab50599-4.jpg)
Overlay histogram showing HeLa cells stained with ab50599 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab50599, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
![All lanes : Anti-alpha Actinin antibody [MAC 276] (ab50599) at 1 µg/mlLane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 2 : Heart (Rat) Tissue Lysate Lane 3 : Skeletal Muscle (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryPeroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/5610_alpha-Actinin-Primary-antibodies-ab50599-7.jpg)
All lanes : Anti-alpha Actinin antibody [MAC 276] (ab50599) at 1 µg/mlLane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 2 : Heart (Rat) Tissue Lysate Lane 3 : Skeletal Muscle (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryPeroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
An equatorial contractile mechanism drives cell elongation but not cell division. - An equatorial contractile mechanism drives cell elongation but not cell division.
Sehring IM, Dong B, Denker E, Bhattachan P, Deng W, Mathiesen BT, Jiang D. PLoS Biol. 2014 Feb 4;12(2):e1001781.
The Drosophila Z-disc protein Z(210) is an adult muscle isoform of Zasp52, which - The Drosophila Z-disc protein Z(210) is an adult muscle isoform of Zasp52, which
Chechenova MB, Bryantsev AL, Cripps RM. J Biol Chem. 2013 Feb 8;288(6):3718-26.
.
Identification and localization of high molecular weight proteins in insect - Identification and localization of high molecular weight proteins in insect
Lakey A, Ferguson C, Labeit S, Reedy M, Larkins A, Butcher G, Leonard K, Bullard B. EMBO J. 1990 Nov;9(11):3459-67.