Anti-Aly antibody [11G5]
| Name | Anti-Aly antibody [11G5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab6141 |
| Prices | $380.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 11G5 |
| Applications | FC IHC-P ICC/IF ICC/IF ELISA WB IP Electron microscopy |
| Species Reactivities | Mouse, Human, Xenopus |
| Antigen | Recombinant human ALY/ REF fusion protein |
| Description | Mouse Monoclonal |
| Gene | ALYREF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab6141 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6141, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab6141 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6141, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Overlay histogram showing HeLa cells stained with ab6141 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6141, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/6296_Aly-Primary-antibodies-ab6141-3.jpg)
Overlay histogram showing HeLa cells stained with ab6141 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6141, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Human TREX component Thoc5 affects alternative polyadenylation site choice by - Human TREX component Thoc5 affects alternative polyadenylation site choice by
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The role of deimination in ATP5b mRNA transport in a transgenic mouse model of - The role of deimination in ATP5b mRNA transport in a transgenic mouse model of
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Differential expression of THOC1 and ALY mRNP biogenesis/export factors in human - Differential expression of THOC1 and ALY mRNP biogenesis/export factors in human
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The cellular DExD/H-box RNA-helicases UAP56 and URH49 exhibit a CRM1-independent - The cellular DExD/H-box RNA-helicases UAP56 and URH49 exhibit a CRM1-independent
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Individual influenza A virus mRNAs show differential dependence on cellular - Individual influenza A virus mRNAs show differential dependence on cellular
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Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export - Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export
Katahira J, Inoue H, Hurt E, Yoneda Y. EMBO J. 2009 Mar 4;28(5):556-67.
FRG1P-mediated aggregation of proteins involved in pre-mRNA processing. - FRG1P-mediated aggregation of proteins involved in pre-mRNA processing.
van Koningsbruggen S, Straasheijm KR, Sterrenburg E, de Graaf N, Dauwerse HG, Frants RR, van der Maarel SM. Chromosoma. 2007 Feb;116(1):53-64. Epub 2006 Nov 14.
The interaction between cap-binding complex and RNA export factor is required for - The interaction between cap-binding complex and RNA export factor is required for
Nojima T, Hirose T, Kimura H, Hagiwara M. J Biol Chem. 2007 May 25;282(21):15645-51. Epub 2007 Mar 15.