Anti-AMPK alpha 1 antibody

• Supplier: Abcam
• Catalog: ab3759
• Prices: $390.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P ICC/IF ICC/IF IP WB
• Species Reactivities: Mouse, Rat, Bovine, Human, Rabbit, Horse, Chicken, Guinea Pig, Dog, Turkey, Pig, Chimpanzee, Monkey, Ape, Fish, Orangutan, Fish, Platypus
• Antigen: Synthetic peptide (Human) - which represented a portion of AMP activated protein Kinase (AMPK), alpha 1 catalytic subunit encoded within exon 8
• Blocking Peptide: Human AMPK alpha 1 peptide
• Description: Rabbit Polyclonal
• Gene: PRKAA1
• Conjugate: Unconjugated

Product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling AMPK alpha 1 with ab3759 at 1/5000 (0.2µg/ml). Detection: DAB.

IHC image of AMPK alpha 1 staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3759, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ICC/IF image of ab3759 stained PANC1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3759, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Mechanisms of resveratrol-induced inhibition of clonal expansion and terminal - Mechanisms of resveratrol-induced inhibition of clonal expansion and terminal

Mitterberger MC, Zwerschke W. J Gerontol A Biol Sci Med Sci. 2013 Nov;68(11):1356-76. doi:

Susceptibility to ATP depletion of primary proximal tubular cell cultures derived - Susceptibility to ATP depletion of primary proximal tubular cell cultures derived

Lieberthal W, Tang M, Zhang L, Viollet B, Patel V, Levine JS. BMC Nephrol. 2013 Nov 14;14:251.

Hypoxia-related protein expression and its clinicopathologic implication in - Hypoxia-related protein expression and its clinicopathologic implication in

Koo JS, Kim H. Tumour Biol. 2011 Oct;32(5):893-904.

AMPK protects proximal tubular cells from stress-induced apoptosis by an - AMPK protects proximal tubular cells from stress-induced apoptosis by an

Lieberthal W, Zhang L, Patel VA, Levine JS. Am J Physiol Renal Physiol. 2011 Dec;301(6):F1177-92. doi:

Adenosine 5'-monophosphate kinase-activated protein kinase (PRKA) activators - Adenosine 5'-monophosphate kinase-activated protein kinase (PRKA) activators

Mayes MA, Laforest MF, Guillemette C, Gilchrist RB, Richard FJ. Biol Reprod. 2007 Apr;76(4):589-97. Epub 2006 Dec 13.

Effects of adenosine monophosphate-activated kinase activators on bovine oocyte - Effects of adenosine monophosphate-activated kinase activators on bovine oocyte

Bilodeau-Goeseels S, Sasseville M, Guillemette C, Richard FJ. Mol Reprod Dev. 2007 Aug;74(8):1021-34.