Anti-Angiotensin Converting Enzyme 2 antibody
| Name | Anti-Angiotensin Converting Enzyme 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab15348 |
| Prices | $400.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Cat, Human, Ferret, Monkey |
| Antigen | Synthetic peptide: CKGENNPGFQNTDDVQTSF , corresponding to C terminal amino acids 788-805 of Human ACE2 |
| Blocking Peptide | Human Angiotensin Converting Enzyme 2 peptide |
| Description | Rabbit Polyclonal |
| Gene | ACE2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab15348 at 2µg/ml staining Angiotensin Converting Enzyme 2 in human kidney by IHC
ICC/IF image of ab15348 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15348, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab15348 staining angiotensin converting enzyme 2 in human thoracic aortic sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized and blocked with a proprietary kit. Samples were incubated with primary antibody (2 µg/ml in TBST + Sodium azide) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.See Abreview
Product References
Angiotensin-converting enzyme 2 decreases formation and severity of angiotensin - Angiotensin-converting enzyme 2 decreases formation and severity of angiotensin
Thatcher SE, Zhang X, Howatt DA, Yiannikouris F, Gurley SB, Ennis T, Curci JA, Daugherty A, Cassis LA. Arterioscler Thromb Vasc Biol. 2014 Dec;34(12):2617-23. doi:
Evidence that angiotensin-(1-7) is an intermediate of gonadotrophin-induced - Evidence that angiotensin-(1-7) is an intermediate of gonadotrophin-induced
Honorato-Sampaio K, Pereira VM, Santos RA, Reis AM. Exp Physiol. 2012 May;97(5):642-50.
Distinct severe acute respiratory syndrome coronavirus-induced acute lung injury - Distinct severe acute respiratory syndrome coronavirus-induced acute lung injury
Smits SL, van den Brand JM, de Lang A, Leijten LM, van Ijcken WF, van Amerongen G, Osterhaus AD, Andeweg AC, Haagmans BL. J Virol. 2011 May;85(9):4234-45.
Discrepancy between intrarenal messenger RNA and protein expression of ACE and - Discrepancy between intrarenal messenger RNA and protein expression of ACE and
Wang G, Lai FM, Lai KB, Chow KM, Kwan CH, Li KT, Szeto CC. Am J Nephrol. 2009;29(6):524-31.
Pathology of experimental SARS coronavirus infection in cats and ferrets. - Pathology of experimental SARS coronavirus infection in cats and ferrets.
van den Brand JM, Haagmans BL, Leijten L, van Riel D, Martina BE, Osterhaus AD, Kuiken T. Vet Pathol. 2008 Jul;45(4):551-62.
Rhesus angiotensin converting enzyme 2 supports entry of severe acute respiratory - Rhesus angiotensin converting enzyme 2 supports entry of severe acute respiratory
Chen Y, Liu L, Wei Q, Zhu H, Jiang H, Tu X, Qin C, Chen Z. Virology. 2008 Nov 10;381(1):89-97.