Anti-AP2 alpha antibody
| Name | Anti-AP2 alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab11828 |
| Prices | $378.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Sheep, Chicken |
| Antigen | Synthetic peptide: SHTDNNAKSSDKEEKHRK conjugated to KLH, corresponding to amino acids 420-437 of Human AP2 alpha |
| Description | Rabbit Polyclonal |
| Gene | TFAP2A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab11828 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11828, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.