Anti-APOA4 antibody [1D4C11]
| Name | Anti-APOA4 antibody [1D4C11] |
|---|---|
| Supplier | Abcam |
| Catalog | ab81616 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | 1D4C11 |
| Applications | ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 21-396 of Human APOA4 with His tag expressed in E |
| Description | Mouse Monoclonal |
| Gene | APOA4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-APOA4 antibody [1D4C11] (ab81616) at 1/500 dilutionLane 1 : truncated APOA4-His recombinant proteinLane 2 : truncated APOA4 (aa21-396)-hIgGFc transfected CHO-K1 cell lysateLane 3 : human serum Lane 4 : human plasma](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/8283_APOA4-Primary-antibodies-ab81616-1.jpg)
All lanes : Anti-APOA4 antibody [1D4C11] (ab81616) at 1/500 dilutionLane 1 : truncated APOA4-His recombinant proteinLane 2 : truncated APOA4 (aa21-396)-hIgGFc transfected CHO-K1 cell lysateLane 3 : human serum Lane 4 : human plasma
ICC/IF image of ab81616 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81616, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.