Flow cytometric analysis of HL-60 cells HL60 cells (right histogram) labeling APOBEC2 with ab175273 at 1/10 dilution compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis
Immunohistochemical analysis of formalin fixed and paraffin embedded mouse heart tissue labeling APOBEC2 with ab175273 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Anti-APOBEC2 antibody - N-terminal (ab175273) at 1/1000 dilution + mouse heart tissue lysate at 15 µgSecondaryHRP anti-rabbit at 1/10000 dilutiondeveloped using the ECL technique