IHC image of Apoliporotein B staining in human liver carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50069, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunoprecipitation of Apolipoprotein B, using a Huh7.5 human hepatoma cell line conditioned medium. The protein was immunoprecipitated from 1.8 mL spent medium, using 29µg of Rabbit polyclonal to Apolipoprotein B (ab50069). A different Apolipoprotein B antibody was used for the western blot. Lanes 1: Pull down with ab50069, Lane 2: IP control with rabbit IgG. Band: >200kDa: Apolipoprotein B.See Abreview