Anti-Apolipoprotein B antibody [EPR2914]

• Supplier: Abcam
• Catalog: ab139401
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR2914
• Applications: FC IP WB
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
• Blocking Peptide: Human Apolipoprotein B peptide
• Description: Rabbit Monoclonal
• Gene: APOB
• Conjugate: Unconjugated

Product images

Overlay histogram showing HepG2 cells stained with ab139401 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab139401, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-Apolipoprotein B antibody [EPR2914] (ab139401) at 1/10000 dilutionLane 1 : Human plasma lysateLane 2 : Human serum lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Western blot analysis on Immunoprecipitation pellet from human serum lysate using anti-Apolipoprotein B RabMAb (ab139401).