Anti-Aquaporin 8 antibody
| Name | Anti-Aquaporin 8 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab77198 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IHC-P FC ELISA |
| Species Reactivities | Human |
| Antigen | Recombinant full length human Aquaporin 8 protein (AAH40630) with a 26 kDa tag |
| Description | Mouse Monoclonal |
| Gene | AQP8 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Aquaporin 8 antibody (ab77198) at 5 µg/ml + IMR-32 cell lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Immunoperoxidase analysis of Aquaporin 8 in formalin-fixed, paraffin-embedded human colon tissue using 3 µg/ml of ab77198.
![Overlay histogram showing HCT116 cells stained with ab77198 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77198, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/8862_Aquaporin-8-Primary-antibodies-ab77198-1.jpg)
Overlay histogram showing HCT116 cells stained with ab77198 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77198, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.