Anti-ARF6 antibody
| Name | Anti-ARF6 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab77581 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Mouse, Rat, Human, Chicken, Bovine, Pig, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus ARF6 |
| Description | Rabbit Polyclonal |
| Gene | ARF6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-ARF6 antibody (ab77581) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 4 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab77581 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77581, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa cells at 1µg/ml.
IHC image of ARF6 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77581, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
All lanes : Anti-ARF6 antibody (ab77581) at 1 µg/mlLane 1 : Lysates prepared from Mouse liver tissue.Lane 2 : Lysates prepared from Mouse lung tissue.Lane 3 : Lysates prepared from Rat liver tissue.Lane 4 : Lysates prepared from Rat lung tissue.Lysates/proteins at 20 µg per lane.SecondaryHRP-conjugated Pig polyclonal to rabbit IgG at 1/5000 dilutionPerformed under reducing conditions.
Product References
Ephrin B1 maintains apical adhesion of neural progenitors. - Ephrin B1 maintains apical adhesion of neural progenitors.
Arvanitis DN, Behar A, Tryoen-Toth P, Bush JO, Jungas T, Vitale N, Davy A. Development. 2013 May;140(10):2082-92.
Chemokine unresponsiveness of chronic lymphocytic leukemia cells results from - Chemokine unresponsiveness of chronic lymphocytic leukemia cells results from
Pye DS, Rubio I, Pusch R, Lin K, Pettitt AR, Till KJ. J Immunol. 2013 Aug 1;191(3):1496-504.
ARF6 directs axon transport and traffic of integrins and regulates axon growth in - ARF6 directs axon transport and traffic of integrins and regulates axon growth in
Eva R, Crisp S, Marland JR, Norman JC, Kanamarlapudi V, ffrench-Constant C, Fawcett JW. J Neurosci. 2012 Jul 25;32(30):10352-64.