Anti-ATP-binding cassette sub-family A member 3 antibody
| Name | Anti-ATP-binding cassette sub-family A member 3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab99856 |
| Prices | $378.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA IHC-P |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Recombinant fragment corresponding to Human ATP-binding cassette sub-family A member 3 aa 38-209 |
| Description | Rabbit Polyclonal |
| Gene | ABCA3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab99856 stained T47D cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99856 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-ATP-binding cassette sub-family A member 3 antibody (ab99856) at 1/1000 dilutionLane 1 : SW480 cell lysateLane 2 : Hela cell lysateLane 3 : Human fetal kidney lysateLane 4 : Human fetal lung lysate