All lanes : Anti-ATP6V1A antibody (ab192355) at 1/2000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysateLane 2 : Raji (Human Burkitt's lymphoma cell line) cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-ATP6V1A antibody (ab192355) at 1/2000 dilution + HepG2 (Human liver hepatocellular carcinoma) cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-ATP6V1A antibody (ab192355) at 1/500 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal kidney lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-ATP6V1A antibody (ab192355) at 1/500 dilutionLane 1 : Mouse brain lysateLane 2 : Rat brain lysateLane 3 : C6 (Rat glial tumor cells) cell lysateLane 4 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysateLane 5 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
ATP6V1A was immunoprecipitated from 1mg of Mouse brain lysate with ab192355 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab192355 at 1/500 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Mouse brain lysate 10 µg (Input). Lane 2: ab192355 IP in Mouse brain lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab192355 in Mouse brain lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
ATP6V1A was immunoprecipitated from 1mg of Mouse brain lysate with ab192355 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab103445 at 1/200 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Mouse brain lysate 10 µg (Input). Lane 2: ab192355 IP in Mouse brain lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab192355 in Mouse brain lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.ab192355 was used to Immunoprecipitate ATPV1A. These precipitates were resolved with ab103445.