Anti-Bag3 antibody

• Supplier: Abcam
• Catalog: ab47124
• Prices: $390.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P IHC-F WB IP
• Species Reactivities: Mouse, Human
• Antigen: A recombinant protein fragment corresponding to the C terminal 196 amino acids of human BAG3
• Description: Rabbit Polyclonal
• Gene: BAG3
• Conjugate: Unconjugated

Product images

All lanes : Anti-Bag3 antibody (ab47124) at 1/2000 dilutionLane 1 : Thymus lysates, total protein (20ug/lane)Lane 2 : Spleen lysates, total protein (20ug/lane)Lane 3 : Skeletal(quadriceps) lysates, total protein (20ug/lane)Lane 4 : Skeletal(diaphragm) lysates, total protein (20ug/lane)Lane 5 : Smooth (heart) muscle lysates, total protein (20ug/lane)

Frozen mouse muscle tissue section stained for BAG3 expression using ab47124 at 1/2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG3 localizes with Z-disk proteins."

Immunohistochemistry of formalin fixed paraffin embedded human pancreas tissue sections using ab47124 at 1/2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.

ICC/IF image of ab47124 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47124, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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