Anti-Bcl-XL antibody
| Name | Anti-Bcl-XL antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2568 |
| Prices | $398.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP WB ICC/IF ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to Bcl-XL |
| Blocking Peptide | Human Bcl-XL peptide |
| Description | Rabbit Polyclonal |
| Gene | BCL2L1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab2568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab2568 staining BCL-XL (green) in Mouse RAW 264.7 cells by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized by 0.1% Triton X-100 in 2% BSA for 15 minutes and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/250 in 2% BSA in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (1/750) was used as the secondary antibody. WGA (red) = Alexa Fluor®568-conjugated Wheat Germ AgglutininSee Abreview
Anti-Bcl-XL antibody (ab2568) at 1/1000 dilution + 25 ug HeLa whole cell lysate at 25 µgSecondaryGoat anti rabbit (HRP) at 1/4000 dilutionPerformed under reducing conditions.
All lanes : Anti-Bcl-XL antibody (ab2568) at 1/1000 dilutionLane 1 : 20ug whole cell lysate from Retinal Pigment EpitheliaLane 2 : 20ug whole cell lysate from MDA MB231 cellsLane 3 : 20ug whole cell lysate from HeLa cellsSecondaryGoat anti-rabbit HRPdeveloped using the ECL techniquePerformed under reducing conditions.
ab2568 used to immunoprecipitate Bcl xL from whole cell lysate of a human Retinal Pigment Epithelial cell line. 7.5ug of antibody was added per mg of lysate and incubated together with Protein A for 1 hour. 500ug of lysate was added per lane. A peroxidase conjugated goat anti-rabbit IgG was used as the secondary antibody.Lane 1: Immunoprecipitated materialLane 2: Control lane (no antibody)This image is courtesy of an Abreview submitted by Ingram Iaccarino on 12 April 2006See Abreview
Product References
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