Anti-Beta Arrestin 2 antibody
| Name | Anti-Beta Arrestin 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54790 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | FC IHC-P WB ICC/IF ICC/IF IP |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment, corresponding to amino acids 300-410 of Human Beta Arrestin 2 |
| Description | Mouse Monoclonal |
| Gene | ARRB2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Beta Arrestin 2 antibody (ab54790) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
Western blot against tagged recombinant protein immunogen using ab54790 Beta Arrestin 2 antibody at 1ug/ml.
ab54790 at 10 ug/ml staining Beta Arrestin 2 in human Hela cells by Immunocytochemistry / Immunofluorescence.
![Overlay histogram showing HeLa cells stained with ab54790 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54790, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was a mix of mouse IgG2a [ICIGG2A], (ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/13801_Beta-Arrestin-2-Primary-antibodies-ab54790-2.jpg)
Overlay histogram showing HeLa cells stained with ab54790 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54790, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was a mix of mouse IgG2a [ICIGG2A], (ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.
Beta Arrestin 2 immunoprecipitation from Human HEK293T cells using ab54790. 1000 µg of cell lysate was incubated with primary antibody (3 µg/mg cell lysate) and matrix (resin) and incubated for 1 hour 30 minutes at 4°C.Lane 1: Whole Cell LysateLane 2: Control IP Lane 3: anti-Beta Arrestin 2 IPSecondary Antibody: Alkaline Phosphatase-conjugated Goat anti-mouse polyclonal (1/1000)See Abreview
Product References
beta-Arrestin interacts with the beta/gamma subunits of trimeric G-proteins and - beta-Arrestin interacts with the beta/gamma subunits of trimeric G-proteins and
Seitz K, Dursch V, Harnos J, Bryja V, Gentzel M, Schambony A. PLoS One. 2014 Jan 29;9(1):e87132.
beta-Arrestin 2 negatively regulates Toll-like receptor 4 (TLR4)-triggered - beta-Arrestin 2 negatively regulates Toll-like receptor 4 (TLR4)-triggered
Li H, Hu D, Fan H, Zhang Y, LeSage GD, Caudle Y, Stuart C, Liu Z, Yin D. J Biol Chem. 2014 Aug 15;289(33):23075-85.
Essential requirement for beta-arrestin2 in mouse intestinal tumors with elevated - Essential requirement for beta-arrestin2 in mouse intestinal tumors with elevated
Bonnans C, Flaceliere M, Grillet F, Dantec C, Desvignes JP, Pannequin J, Severac D, Dubois E, Bibeau F, Escriou V, Crespy P, Journot L, Hollande F, Joubert D. Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):3047-52. doi:
Cross talk between phosphatidylinositol 3-kinase and cyclic AMP (cAMP)-protein - Cross talk between phosphatidylinositol 3-kinase and cyclic AMP (cAMP)-protein
Bjorgo E, Solheim SA, Abrahamsen H, Baillie GS, Brown KM, Berge T, Okkenhaug K, Houslay MD, Tasken K. Mol Cell Biol. 2010 Apr;30(7):1660-72.