Anti-beta Catenin (phospho Y142) antibody
| Name | Anti-beta Catenin (phospho Y142) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab27798 |
| Prices | $398.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | The antibody detects beta Catenin on SDS PAGE immunoblots of Hct116 src transformed cells treated with pervanadate, but not in control cells |
| Blocking Peptide | Human beta Catenin (phospho Y142) peptide |
| Description | Rabbit Polyclonal |
| Gene | CTNNB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF labeling of phosphorylated β-Catenin in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal β-Catenin (left panel) and ab27798 antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
ab27798 staining beta Catenin (phospho Y142) in rat hippocampal neurons by Immunocytochemistry/ Immunofluorescence. The cells were pre-treated with pervanadate for 15 minutes before being fixed in paraformaldehyde and then blocked using 10% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/50 for 8 hours at 4°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 594 (red) ab150076) used at a 1/500 dilution. Hoestch was used to stain the cell nuclei (blue).
Lanes 1 - 2 : anti beta cateninLanes 3 - 4 : Anti-beta Catenin (phospho Y142) antibody (ab27798) at 1/500 dilutionLane 1 : Hct116 src transformed cells serum starved overnight.Lane 2 : Hct116 src transformed cells treated with pervanadate (1 mM) for 30 min.Lane 3 : Hct116 src transformed cells serum starved overnight.Lane 4 : Hct116 src transformed cells treated with pervanadate (1 mM) for 30 min.Lysates/proteins at 20 µg per lane.
Product References
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Mishra J, Verma RK, Alpini G, Meng F, Kumar N. J Biol Chem. 2013 Nov 1;288(44):31795-806.
The endothelin A receptor and epidermal growth factor receptor signaling converge - The endothelin A receptor and epidermal growth factor receptor signaling converge
Cianfrocca R, Tocci P, Spinella F, Di Castro V, Bagnato A, Rosano L. Life Sci. 2012 Oct 15;91(13-14):550-6.
Calpain induces N-terminal truncation of beta-catenin in normal murine liver - Calpain induces N-terminal truncation of beta-catenin in normal murine liver
Lade A, Ranganathan S, Luo J, Monga SP. J Biol Chem. 2012 Jun 29;287(27):22789-98.
Lung cancer cell migration is regulated via repressing growth factor PTN/RPTP - Lung cancer cell migration is regulated via repressing growth factor PTN/RPTP
Feng ZJ, Gao SB, Wu Y, Xu XF, Hua X, Jin GH. Oncogene. 2010 Sep 30;29(39):5416-26.
RET/PTC1-driven neoplastic transformation and proinvasive phenotype of human - RET/PTC1-driven neoplastic transformation and proinvasive phenotype of human
Cassinelli G, Favini E, Degl'Innocenti D, Salvi A, De Petro G, Pierotti MA, Zunino F, Borrello MG, Lanzi C. Neoplasia. 2009 Jan;11(1):10-21.
Epidermal growth factor receptor/beta-catenin/T-cell factor 4/matrix - Epidermal growth factor receptor/beta-catenin/T-cell factor 4/matrix
Jean C, Blanc A, Prade-Houdellier N, Ysebaert L, Hernandez-Pigeon H, Al Saati T, Haure MJ, Coluccia AM, Charveron M, Delabesse E, Laurent G. Cancer Res. 2009 Apr 15;69(8):3291-9.
Molecular characterization of synovial sarcoma in children and adolescents: - Molecular characterization of synovial sarcoma in children and adolescents:
Bozzi F, Ferrari A, Negri T, Conca E, Luca da R, Losa M, Casieri P, Orsenigo M, Lampis A, Meazza C, Casanova M, Pierotti MA, Tamborini E, Pilotti S. Transl Oncol. 2008 Jul;1(2):95-101.
Unique mechanisms of growth regulation and tumor suppression upon Apc - Unique mechanisms of growth regulation and tumor suppression upon Apc
Strom A, Bonal C, Ashery-Padan R, Hashimoto N, Campos ML, Trumpp A, Noda T, Kido Y, Real FX, Thorel F, Herrera PL. Development. 2007 Aug;134(15):2719-25. Epub 2007 Jun 27.