Anti-BMAL1 antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab3350
• Prices: $391.00
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IP EMSA ChIP
• Species Reactivities: Mouse, Rat, Hamster, Human, Horse, Chicken
• Antigen: Synthetic peptide corresponding to Mouse BMAL1 aa 582-594
• Description: Rabbit Polyclonal
• Gene: ARNTL
• Conjugate: Unconjugated

Product images

All lanes : Anti-BMAL1 antibody - ChIP Grade (ab3350) at 1/500 dilutionLane 1 : U251 cell lysateLane 2 : U87-MG cell lysateLane 3 : NIH-3T3 cell lysateLysates/proteins at 25 µg per lane.

Anti-BMAL1 antibody - ChIP Grade (ab3350) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL technique

ICC/IF image of ab3350 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3350, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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Bmal1 and beta-cell clock are required for adaptation to circadian disruption, - Bmal1 and beta-cell clock are required for adaptation to circadian disruption,

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