Anti-BMP2 antibody [65529.111]
| Name | Anti-BMP2 antibody [65529.111] |
|---|---|
| Supplier | Abcam |
| Catalog | ab6285 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 65529.111 |
| Applications | WB ELISA IHC-P ICC/IF ICC/IF ELISA |
| Species Reactivities | Mouse, Rat, Rabbit, Human |
| Antigen | Recombinant full length protein expressed in E |
| Description | Mouse Monoclonal |
| Gene | BMP2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab66285 staining BMP2 in Human small intestine tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1.5% serum for 10 minutes. Antigen retrieval was by heat mediation in citrate (pH6.0). Samples were incubated with primary antibody (1/100 in diluent buffer) for 1 hour. An undiluted HRP-conjugated Goat polyclonal to mouse IgG was used as secondary antibodySee Abreview
ICC/IF image of ab6285 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6285, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
The effect of electroacupuncture on osteotomy gap healing in a rat fibula model. - The effect of electroacupuncture on osteotomy gap healing in a rat fibula model.
Inoue M, Nakajima M, Hojo T, Itoi M, Kitakoji H. Acupunct Med. 2013 Jun;31(2):222-7.
Bone resorption and remodeling in murine collagenase-induced osteoarthritis after - Bone resorption and remodeling in murine collagenase-induced osteoarthritis after
Ivanovska N, Dimitrova P. Arthritis Res Ther. 2011 Mar 16;13(2):R44.
Osteoinduction of hydroxyapatite/beta-tricalcium phosphate bioceramics in mice - Osteoinduction of hydroxyapatite/beta-tricalcium phosphate bioceramics in mice
Cheng L, Ye F, Yang R, Lu X, Shi Y, Li L, Fan H, Bu H. Acta Biomater. 2010 Apr;6(4):1569-74.
Expression of gremlin, a bone morphogenetic protein antagonist,is associated with - Expression of gremlin, a bone morphogenetic protein antagonist,is associated with
Jara A, Chacon C, Burgos ME, Droguett A, Valdivieso A, Ortiz M, Troncoso P, Mezzano S. Nephrol Dial Transplant. 2009 Apr;24(4):1121-9.
Constitutively active Akt induces ectodermal defects and impaired bone - Constitutively active Akt induces ectodermal defects and impaired bone
Segrelles C, Moral M, Lorz C, Santos M, Lu J, Cascallana JL, Lara MF, Carbajal S, Martinez-Cruz AB, Garcia-Escudero R, Beltran L, Segovia JC, Bravo A, DiGiovanni J, Paramio JM. Mol Biol Cell. 2008 Jan;19(1):137-49. Epub 2007 Oct 24.
Cross-talk between bone morphogenetic protein and transforming growth factor-beta - Cross-talk between bone morphogenetic protein and transforming growth factor-beta
Yew KH, Hembree M, Prasadan K, Preuett B, McFall C, Benjes C, Crowley A, Sharp S, Tulachan S, Mehta S, Tei E, Gittes G. J Biol Chem. 2005 Sep 16;280(37):32209-17. Epub 2005 Jul 14.