Anti-BMP4 antibody
| Name | Anti-BMP4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab39973 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P IHC-F ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Bovine, Pig |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BMP4 |
| Blocking Peptide | Human BMP4 peptide |
| Description | Rabbit Polyclonal |
| Gene | BMP4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-BMP4 antibody (ab39973) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Mouse) Tissue LysateLane 3 : Brain (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
All lanes : Anti-BMP4 antibody (ab39973) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Mouse) Mouse Tissue Lysate Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 4 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 5 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 6 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466) with Human BMP4 peptide (ab40140) at 1 µg/mlLane 7 : Brain (Mouse) Mouse Tissue Lysate with Human BMP4 peptide (ab40140) at 1 µg/mlLane 8 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889) with Human BMP4 peptide (ab40140) at 1 µg/mlLane 9 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431) with Human BMP4 peptide (ab40140) at 1 µg/mlLane 10 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) with Human BMP4 peptide (ab40140) at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti
All lanes : Anti-BMP4 antibody (ab39973) at 1/1000 dilutionLane 1 : mouse brainLane 2 : mouse brainLane 3 : mouse brainLane 4 : mouse brainLysates/proteins at 20 µg per lane.SecondaryDako Cytomation P0260 Conjugation: HRP at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab39973 staining primary hippocampal neurons. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39973, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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