Anti-BMP5 antibody (ab88064) at 5 µg/ml + HeLa cells nuclear extract at 50 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
ICC/IF image of ab88064 staining of BMP5 in rat hippocampal culture. The sections were incubated in 0.3% triton X to permeabilise the cells and then in 10% serum to block non-specific protein-protein interactions. A two hour incubation at +24°C was performed with ab88064 (1:1000), followed by Alexa Fluor 568 conjugated secondary antibody. Red staining of the hippocampal neurons was observed.See Abreview
Detection limit for recombinant tagged BMP5 is approximately 1ng/ml as a capture antibody.