Anti-BMP7 antibody
| Name | Anti-BMP7 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56023 |
| Prices | $398.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Sheep, Rabbit, Horse, Guinea Pig, Bovine, Cat, Dog, Pig |
| Antigen | Synthetic peptide: QGKHNSAPMF MLDLYNAMAV EEGGGPGGQG FSYPYKAVFS TQGPPLASLQ, corresponding to N terminal amino acids 73-122 of Human BMP7 |
| Blocking Peptide | Human BMP7 peptide |
| Description | Rabbit Polyclonal |
| Gene | BMP7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling BMP7 with ab56023 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
Lane 1 : Anti-BMP7 antibody (ab56023) at 1.25 µg/mlLane 2 : Anti-BMP7 antibody (ab56023) at 2.5 µg/mlLane 3 : Anti-BMP7 antibody (ab56023) at 5 µg/mlLane 1 : Cell lysate prepared from BMP7 transfected 293T cellsLane 2 : Cell lysate prepared from BMP7 transfected 293T cellsLane 3 : Cell lysate prepared from BMP7 transfected 293T cellsLysates/proteins at 25 µg per lane.
ICC/IF image of ab56023 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56023, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-BMP7 antibody (ab56023) at 1/500 dilutionLane 1 : Rat Brain Whole Tissue (age P8) Lysate Lane 2 : Rat Brain Whole Tissue (age P15) Lysate Lane 3 : Rat Brain Whole Tissue (age P21) Lysate Lane 4 : Rat Brain Whole Tissue (age P21) Lysate Lysates/proteins at 20 µg per lane.SecondaryHRP conjugated at 1/3000 dilutionPerformed under reducing conditions.
ab56023 staining mouse e18 skeleton tissues by immunohistochemistry (frozen sections). Sections were formaldehyde fixed prior to being blocked in 20% serum for 1 hour at RT. Sample was incubated with ab56023, diluted 1/1000, for 16 hours at 4°C. Alexa fluor® 680 donkey polyclonal, diluted 1/200, was used as the secondary antibody.See Abreview
Product References
Putative prognostic epithelial-to-mesenchymal transition biomarkers for - Putative prognostic epithelial-to-mesenchymal transition biomarkers for
Whiteland H, Spencer-Harty S, Thomas DH, Davies C, Morgan C, Kynaston H, Bose P, Fenn N, Lewis PD, Bodger O, Jenkins S, Doak SH. Exp Mol Pathol. 2013 Oct;95(2):220-6.
Effects of demineralized bone matrix on tendon-bone healing in an intra-articular - Effects of demineralized bone matrix on tendon-bone healing in an intra-articular
Lovric V, Chen D, Yu Y, Oliver RA, Genin F, Walsh WR. Am J Sports Med. 2012 Oct;40(10):2365-74. Epub 2012 Sep 14.
Bone morphogenetic protein-7 is a MYC target with prosurvival functions in - Bone morphogenetic protein-7 is a MYC target with prosurvival functions in
Fiaschetti G, Castelletti D, Zoller S, Schramm A, Schroeder C, Nagaishi M, Stearns D, Mittelbronn M, Eggert A, Westermann F, Ohgaki H, Shalaby T, Pruschy M, Arcaro A, Grotzer MA. Oncogene. 2011 Jun 23;30(25):2823-35.
Elastomeric osteoconductive synthetic scaffolds with acquired osteoinductivity - Elastomeric osteoconductive synthetic scaffolds with acquired osteoinductivity
Filion TM, Li X, Mason-Savas A, Kreider JM, Goldstein SA, Ayers DC, Song J. Tissue Eng Part A. 2011 Feb;17(3-4):503-11.
Expression of chondro-osteogenic BMPs in clinical samples of patellar - Expression of chondro-osteogenic BMPs in clinical samples of patellar
Rui YF, Lui PP, Rolf CG, Wong YM, Lee YW, Chan KM. Knee Surg Sports Traumatol Arthrosc. 2012 Jul;20(7):1409-17. doi:
Combination of small molecules enhances differentiation of mouse embryonic stem - Combination of small molecules enhances differentiation of mouse embryonic stem
Mae S, Shirasawa S, Yoshie S, Sato F, Kanoh Y, Ichikawa H, Yokoyama T, Yue F, Tomotsune D, Sasaki K. Biochem Biophys Res Commun. 2010 Mar 19;393(4):877-82. doi:
Regulation of embryonic kidney branching morphogenesis and glomerular development - Regulation of embryonic kidney branching morphogenesis and glomerular development
Yi T, Tan K, Cho SG, Wang Y, Luo J, Zhang W, Li D, Liu M. J Biol Chem. 2010 Jun 4;285(23):17811-20.
Bone morphogenetic protein 7 is expressed in prostate cancer metastases and its - Bone morphogenetic protein 7 is expressed in prostate cancer metastases and its
Morrissey C, Brown LG, Pitts TE, Vessella RL, Corey E. Neoplasia. 2010 Feb;12(2):192-205.