Anti-BMP7 antibody
| Name | Anti-BMP7 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab27569 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB ELISA ICC/IF ICC/IF B/N |
| Species Reactivities | Mouse, Rat, Human, Xenopus |
| Antigen | Recombinant full length human BMP7 protein |
| Description | Rabbit Polyclonal |
| Gene | BMP7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab27569 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27569, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ICC/IF image of Rat primary hepatocytes culture cells stained with ab27569. The cells were fixed with 4% PFA and incubated in 5% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27569, 1µg/ml) and (ab15640) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 568 (red) at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Neuroprotection by rAAV-mediated gene transfer of bone morphogenic protein 7. - Neuroprotection by rAAV-mediated gene transfer of bone morphogenic protein 7.
Heinonen AM, Rahman M, Dogbevia G, Jakobi H, Wolfl S, Sprengel R, Schwaninger M. BMC Neurosci. 2014 Mar 11;15:38.
Allelic depletion of grem1 attenuates diabetic kidney disease. - Allelic depletion of grem1 attenuates diabetic kidney disease.
Roxburgh SA, Kattla JJ, Curran SP, O'Meara YM, Pollock CA, Goldschmeding R, Godson C, Martin F, Brazil DP. Diabetes. 2009 Jul;58(7):1641-50.
Targeted repression of bone morphogenetic protein 7, a novel target of the p53 - Targeted repression of bone morphogenetic protein 7, a novel target of the p53
Yan W, Chen X. Cancer Res. 2007 Oct 1;67(19):9117-24.