IHC image of TBMPR1A staining in Normal human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab174815, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-BMPR1A antibody (ab174815) at 1 µg/mlLane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell LysateLane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 1 µg/ml per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.