Anti-BMPR2 antibody

• Supplier: Abcam
• Catalog: ab96826
• Prices: $378.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Human, Rat
• Antigen: Recombinant fragment containing a sequence corresponding to a region within amino acids 667-921 of Human BMPR2 (NP_001195)
• Description: Rabbit Polyclonal
• Gene: BMPR2
• Conjugate: Unconjugated

Product images

ICC/IF image of ab96826 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96826 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

All lanes : Anti-BMPR2 antibody (ab96826) at 1/1000 dilutionLane 1 : H1299 whole cell lysateLane 2 : Raji whole cell lysateLysates/proteins at 30 µg per lane.

Anti-BMPR2 antibody (ab96826) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

Product References

Bone morphogenetic protein 4 promotes mammalian oogonial stem cell - Bone morphogenetic protein 4 promotes mammalian oogonial stem cell

Park ES, Woods DC, Tilly JL. Fertil Steril. 2013 Nov;100(5):1468-75.

Growth differentiation factor 5 is a key physiological regulator of dendrite - Growth differentiation factor 5 is a key physiological regulator of dendrite

Osorio C, Chacon PJ, Kisiswa L, White M, Wyatt S, Rodriguez-Tebar A, Davies AM. Development. 2013 Dec;140(23):4751-62.