Anti-Bub3 antibody [EPR5319(2)] (Alexa Fluor® 488)
| Name | Anti-Bub3 antibody [EPR5319(2)] (Alexa Fluor® 488) |
|---|---|
| Supplier | Abcam |
| Catalog | ab200803 |
| Prices | $425.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR5319(2) |
| Applications | ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Bub3 (N terminal) |
| Description | Rabbit Monoclonal |
| Gene | BUB3 |
| Conjugate | Alexa Fluor® 488 |
| Supplier Page | Shop |
Product images
ab200803 staining Bub3 in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200803 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).