Human SPARC Affinity Purified Polyclonal Ab, Goat IgG
| Name | Human SPARC Affinity Purified Polyclonal Ab, Goat IgG |
|---|---|
| Supplier | R&D Systems |
| Catalog | AF941-SP |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB FC IHC Simple Western |
| Species Reactivities | Human |
| Antigen | Mouse myeloma cell line NS0-derived recombinant human SPARC/Osteonectin. Ala18-Ile303 Accession Number P09486 |
| Description | Goat Polyclonal |
| Gene | SPARC |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Detection of Human SPARC by Western Blot. Western blot shows lysates of MG‑63 human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF941) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SPARC at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SPARC/Osteonectin in Human Ovary. SPARC/Osteonectin was detected in immersion fixed paraffin-embedded sections of human ovary using Goat Anti-Human SPARC/Osteonectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF941) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human SPARC by Simple WesternTM. Simple Western lane view shows lysates of MG‑63 human osteosarcoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SPARC at approximately 57 kDa (as indicated) using 20 µg/mL of Goat Anti-Human SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF941) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Product References
SPARC is a key regulator of proliferation, apoptosis and invasion in human - SPARC is a key regulator of proliferation, apoptosis and invasion in human
Chen J, Wang M, Xi B, Xue J, He D, Zhang J, Zhao Y. PLoS One. 2012;7(8):e42413.
Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer - Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer
Chen J, Shi D, Liu X, Fang S, Zhang J, Zhao Y. BMC Cancer. 2012 Oct 10;12:464.
Diverse functions of reactive cysteines facilitate unique biosynthetic processes - Diverse functions of reactive cysteines facilitate unique biosynthetic processes
Shen M, Siu S, Byrd S, Edelmann KH, Patel N, Ketchem RR, Mehlin C, Arnett HA, Hasegawa H. Exp Cell Res. 2011 Apr 15;317(7):976-93.
SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration. - SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R. J Leukoc Biol. 2007 Mar;81(3):748-56. Epub 2006 Dec 18.