Anti-c-Myc antibody [9E10] - ChIP Grade
| Name | Anti-c-Myc antibody [9E10] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab32 |
| Prices | $404.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 9E10 |
| Applications | ICC/IF ICC/IF ICC/IF ChIP IHC FC WB IP ELISA IHC-P IHC-F Purification |
| Species Reactivities | Mouse, Human, Drosophila |
| Antigen | Synthetic peptide: AEEQKLISEE DLLRKRREQL KHKLE conjugated to KLH, corresponding to C terminal amino acids 408-432 of Human c-Myc |
| Description | Mouse Monoclonal |
| Gene | MYC |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-c-Myc antibody [9E10] - ChIP Grade (ab32) at 1 µg/ml + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 10 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/16481_c-Myc-Primary-antibodies-ab32-15.jpg)
Anti-c-Myc antibody [9E10] - ChIP Grade (ab32) at 1 µg/ml + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 10 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Overlay histogram showing Jurkat cells stained with ab32 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/16482_c-Myc-Primary-antibodies-ab32-39.jpg)
Overlay histogram showing Jurkat cells stained with ab32 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ICC/IF image of ab32 stained human Hek293 cells. The cells were methanol fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab32 staining c-Myc in HEK293 cells transfected with pCMV4-myc-Mfn2 by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 5% horse serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/1200 in 5% horse serum) for 1 hour. An AlexaFluor®488-conjugated goat anti-mouse polyclonal IgG (1/400) was used as the secondary antibody. See Abreview
Product References
Canine epidermal neural crest stem cells: characterization and potential as - Canine epidermal neural crest stem cells: characterization and potential as
Gericota B, Anderson JS, Mitchell G, Borjesson DL, Sturges BK, Nolta JA, Sieber-Blum M. Stem Cells Transl Med. 2014 Mar;3(3):334-45.
The diabetes gene Hhex maintains delta-cell differentiation and islet function. - The diabetes gene Hhex maintains delta-cell differentiation and islet function.
Zhang J, McKenna LB, Bogue CW, Kaestner KH. Genes Dev. 2014 Apr 15;28(8):829-34.
c-Myc-mediated repression of miR-15-16 in hypoxia is induced by increased - c-Myc-mediated repression of miR-15-16 in hypoxia is induced by increased
Xue G, Yan HL, Zhang Y, Hao LQ, Zhu XT, Mei Q, Sun SH. Oncogene. 2015 Mar 12;34(11):1393-406.
SIRT1 mediates FOXA2 breakdown by deacetylation in a nutrient-dependent manner. - SIRT1 mediates FOXA2 breakdown by deacetylation in a nutrient-dependent manner.
van Gent R, Di Sanza C, van den Broek NJ, Fleskens V, Veenstra A, Stout GJ, Brenkman AB. PLoS One. 2014 May 29;9(5):e98438.
PP2A/B55 and Fcp1 regulate Greatwall and Ensa dephosphorylation during mitotic - PP2A/B55 and Fcp1 regulate Greatwall and Ensa dephosphorylation during mitotic
Hegarat N, Vesely C, Vinod PK, Ocasio C, Peter N, Gannon J, Oliver AW, Novak B, Hochegger H. PLoS Genet. 2014 Jan;10(1):e1004004.
Mechanism of action of phenethylisothiocyanate and other reactive oxygen - Mechanism of action of phenethylisothiocyanate and other reactive oxygen
Jutooru I, Guthrie AS, Chadalapaka G, Pathi S, Kim K, Burghardt R, Jin UH, Safe S. Mol Cell Biol. 2014 Jul;34(13):2382-95.
The histone variant composition of centromeres is controlled by the pericentric - The histone variant composition of centromeres is controlled by the pericentric
Boyarchuk E, Filipescu D, Vassias I, Cantaloube S, Almouzni G. J Cell Sci. 2014 Aug 1;127(Pt 15):3347-59.
The 9-1-1 checkpoint clamp stimulates DNA resection by Dna2-Sgs1 and Exo1. - The 9-1-1 checkpoint clamp stimulates DNA resection by Dna2-Sgs1 and Exo1.
Ngo GH, Balakrishnan L, Dubarry M, Campbell JL, Lydall D. Nucleic Acids Res. 2014;42(16):10516-28.
POT1 mutations cause telomere dysfunction in chronic lymphocytic leukemia. - POT1 mutations cause telomere dysfunction in chronic lymphocytic leukemia.
Ramsay AJ, Quesada V, Foronda M, Conde L, Martinez-Trillos A, Villamor N, Rodriguez D, Kwarciak A, Garabaya C, Gallardo M, Lopez-Guerra M, Lopez-Guillermo A, Puente XS, Blasco MA, Campo E, Lopez-Otin C. Nat Genet. 2013 May;45(5):526-30.
The ING4 Binding with p53 and Induced p53 Acetylation were Attenuated by Human - The ING4 Binding with p53 and Induced p53 Acetylation were Attenuated by Human
Guo Y, Meng X, Wang Q, Wang Y, Shang H. PLoS One. 2013 Aug 13;8(8):e71453.