Western blot analysis of extracts from Saos-2 and LNCaP cells using RUNX2 (D1L7F) Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457.
Immunoprecipitation of RUNX2 from Saos-2 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or RUNX2 (D1L7F) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using RUNX2 (D1L7F) Rabbit mAb.
Confocal immunofluorescent analysis of Saos-2 (left) and LNCaP (right) cells using RUNX2 (D1L7F) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Flow cytometric analysis of LNCaP (blue) and Saos-2 (green) cells using RUNX2 (D1L7F) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 Saos-2 cells and either 10 μl of RUNX2 (D1L7F) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human RUNX1 Intron 1 primers, SimpleChIP ® Human RUNX2 Promoter Primers #12376, human FRA10AC1 intron 1 primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.