Anti-CACNB2 antibody [S8b-1]
| Name | Anti-CACNB2 antibody [S8b-1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab93606 |
| Prices | $387.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | S8b-1 |
| Applications | WB IHC-P IHC-F ICC/IF ICC/IF FC IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide: RSPKPSANSVTSPHSKE , corresponding to internal sequence amino acids 189 - 205 of Rat CACNB2 (NP_446303) |
| Description | Mouse Monoclonal |
| Gene | CACNB2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of ab93606 staining in Human Heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93606, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Overlay histogram showing SH-SY5Y cells stained with ab93606 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93606, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/19042_CACNB2-Primary-antibodies-ab93606-2.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab93606 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93606, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
CACNB2 immunoprecipitation from mouse skeletal muscle tissue using ab93606. 500 µg of cell lysate was incubated with undiluted primary antibody and matrix (Protein G) and incubated for 12 hours at 4°C.Lane 1: Mouse skeletal muscle lysateLane 2: anti-CACNB2 IP Western blot detection: ab93606 at 1/500 dilutionSee Abreview
Product References
Identification of new dystroglycan complexes in skeletal muscle. - Identification of new dystroglycan complexes in skeletal muscle.
Johnson EK, Li B, Yoon JH, Flanigan KM, Martin PT, Ervasti J, Montanaro F. PLoS One. 2013 Aug 8;8(8):e73224.
A short polybasic segment between the two conserved domains of the beta2a-subunit - A short polybasic segment between the two conserved domains of the beta2a-subunit
Miranda-Laferte E, Schmidt S, Jara AC, Neely A, Hidalgo P. J Biol Chem. 2012 Sep 21;287(39):32588-97. Epub 2012 Jul 31.