Detection of Mouse and Rat Testisin/Prss21 by Western Blot. Western blot shows lysates of mouse testis tissue and rat testis tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Testisin/Prss21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6820) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017 ). A specific band was detected for Testisin/Prss21 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
Testisin/Prss21 in Mouse Testis. Testisin/Prss21 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse Testisin/Prss21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6820) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008 ) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of spermatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections . This application has not been tested in rat samples.