Anti-Calnexin antibody [AF18] - ER Membrane Marker
| Name | Anti-Calnexin antibody [AF18] - ER Membrane Marker |
|---|---|
| Supplier | Abcam |
| Catalog | ab31290 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | AF18 |
| Applications | FC ICC/IF ICC/IF IP WB IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Focus cells (human hepatoma cell line) |
| Description | Mouse Monoclonal |
| Gene | CANX |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-Calnexin antibody [AF18] - ER Membrane Marker (ab31290) at 5 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab52255)Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab52256)Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/19562_ab31290-238537-WBAP16900771.jpg)
All lanes : Anti-Calnexin antibody [AF18] - ER Membrane Marker (ab31290) at 5 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab52255)Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab52256)Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab31290 stained human HeLa cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab31290, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
IHC image of Calnexin staining in human liver carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31290, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
![Overlay histogram showing HeLa cells stained with ab31290 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab31290, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/19565_Calnexin--ER-membrane-marker-Primary-antibodies-ab31290-3.jpg)
Overlay histogram showing HeLa cells stained with ab31290 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab31290, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
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