All lanes : Anti-Carbonic Anhydrase I antibody (ab92649) at 1/500 dilutionLane 1 : Extracts from K562 cells (without competing peptide)Lane 2 : Extracts from K562 cells (with competing peptide)Lysates/proteins at 5 µg per lane.
ab92649 at 1/50 dilution staining Carbonic Anhydrase I in paraffin-embedded human colon carcinoma tissue (1) without competing peptide and (2) with competing peptide, by immunohistochemistry.
Anti-Carbonic Anhydrase I antibody (ab92649) at 1/500 dilution + Human Carbonic Anhydrase I full length protein (ab89359) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 20 seconds