Anti-Cardiac Troponin T antibody
| Name | Anti-Cardiac Troponin T antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab64623 |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP ICC/IF ICC/IF WB ELISA IHC-F IHC-P |
| Species Reactivities | Human |
| Antigen | Human cardiac troponin T, purity more than 98% |
| Description | Goat Polyclonal |
| Gene | TNNT2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of Cardiac Troponin T staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64623, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemical analysis of transverse cryosections of uninjured newt heart tissue (ventricle), staining Cardiac Troponin T with ab64623. Sections were fixed with 4% formaldehyde and blocked with 10% swine serum. Samples were incubated with primary antibody overnight at 4°C and an AlexaFluor®488-conjugated anti-goat IgG was used as the secondary antibody.
Product References
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