Anti-Casein Kinase 2 beta antibody [EP1995Y]

• Supplier: Abcam
• Catalog: ab76025
• Prices: $395.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1995Y
• Applications: WB FC IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Horse, Human
• Antigen: Synthetic peptide corresponding to residues near the C terminus of human Casein Kinase 2 beta protein
• Description: Rabbit Monoclonal
• Gene: CSNK2B
• Conjugate: Unconjugated

Product images

Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + K562 cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunofluorescent staining of Casein Kinase 2 beta in HeLa cells using ab76025 at a 1/100 dilution.

Immunohistochemical analysis of Casein Kinase 2 beta in paraffin embedded human ovary carcinoma tissue using ab76025 at a 1/100 dilution.

Overlay histogram showing HeLa cells stained with ab76025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76025, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.